Sucrose hemolysis test; Hemolytic anemia sugar water hemolysis test; Paroxysmal nocturnal hemoglobinuria sugar water hemolysis test; PNH sugar water hemolysis test
The sugar-water hemolysis test is a blood test to detect fragile red blood cells. It does this by testing how well they withstand swelling in a sugar (sucrose) solution.
A blood sample is needed.
There is no special preparation needed for this test.
When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing or a slight bruise. This soon goes away.
Your health care provider may recommend this test if you have signs or symptoms of paroxysmal nocturnal hemoglobinuria (PNH) or hemolytic anemia of unknown cause. Hemolytic anemia is a condition in which red blood cells die before they should. PNH red blood cells are very likely to be harmed by the body's complement system. The complement system is proteins that move through the bloodstream. These proteins work with the immune system.
A normal test result is called a negative result. A normal result shows that less than 5% of red blood cells break down when tested. This breakdown is called hemolysis.
A negative test does not rule out PNH. False-negative results may occur if the fluid part of blood (serum) lacks complement.
Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your provider about the meaning of your specific test results.
A positive test result means the results are abnormal. In a positive test, more than 10% of red blood cells break down. It could indicate the person has PNH.
Certain conditions can make the test results appear positive (called "false positive"). These conditions are autoimmune hemolytic anemias and leukemia.
There is little risk involved with having your blood taken. Veins and arteries vary in size from one person to another and from one side of the body to the other. Taking blood from some people may be more difficult than from others.
Other risks associated with having blood drawn are slight, but may include:
Brodsky RA. Paroxysmal nocturnal hemoglobinuria. In: Hoffman R, Benz EJ, Silberstein LE, eds. Hematology: Basic Principles and Practice. 7th ed. Philadelphia, PA: Elsevier; 2018:chap 31.
Chernecky CC, Berger BJ. Sucrose hemolysis test - diagnostic. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. St Louis, MO: Elsevier Saunders; 2013:1050.
Gallagher PG. Hemolytic anemias: red blood cell membrane and metabolic defects. In: Goldman L, Schafer AI, eds. Goldman-Cecil Medicine. 26th ed. Philadelphia, PA: Elsevier; 2020:chap152.BACK TO TOP
Review Date: 2/6/2020
Reviewed By: Todd Gersten, MD, Hematology/Oncology, Florida Cancer Specialists & Research Institute, Wellington, FL. Review provided by VeriMed Healthcare Network. Also reviewed by David Zieve, MD, MHA, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.
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