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25-hydroxy vitamin D test

25-OH vitamin D test; Calcidiol; 25-hydroxycholecalciferol test

The 25-hydroxy vitamin D test is the most accurate way to measure how much vitamin D is in your body.

Vitamin D helps control calcium and phosphate levels in the body.

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Blood test

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How the Test is Performed

A blood sample is needed.

How to Prepare for the Test

Usually, you will not need to fast. But this depends on the laboratory and the testing method used. Follow any instructions for not eating before the test.

How the Test will Feel

When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing.

Why the Test is Performed

This test is done to determine if you have too much or too little vitamin D in your blood. Screening of all adults, even when pregnant, for low vitamin D levels is generally not recommended.

However, screening may be done on people who are at high risk for vitamin D deficiency, such as those who:

Normal Results

The normal range of vitamin D is measured as nanograms per milliliter (ng/mL). Many experts recommend a level between 20 and 40 ng/mL. Others recommend a level between 30 and 50 ng/mL.

The examples above are common measurements for results of these tests. Normal value ranges may vary slightly among different laboratories. Some laboratories use different measurements or test different samples. Talk to your doctor about the meaning of your specific test results, and whether you may need vitamin D supplements.

Many people are confused by the way these tests are reported.

What Abnormal Results Mean

A lower-than-normal level can be due to a vitamin D deficiency, which can result from:

A low vitamin D level is more common in African American children (especially in the winter), as well as in infants who are breastfed only.

A higher-than-normal level may be due to excess vitamin D, a condition called hypervitaminosis D. This is most commonly caused by taking too much vitamin D. It can result in too much calcium in the body (hypercalcemia). This leads to many symptoms and kidney damage.

Risks

There is little risk involved with having your blood taken. Veins and arteries vary in size from one person to another and from one side of the body to the other. Taking blood from some people may be more difficult than from others.

Other risks associated with having blood drawn are slight, but may include:

Related Information

Hyperparathyroidism
Hypervitaminosis D
Hypercalcemia

References

Bouillon R. Vitamin D: from photosynthesis, metabolism, and action to clinical applications. In: Jameson JL, De Groot LJ, de Kretser DM, et al, eds. Endocrinology: Adult and Pediatric. 7th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 59.

Chernecky CC, Berger BJ. Vitamin D (cholecalciferol) -- plasma or serum. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. St Louis, MO: Elsevier Saunders; 2013:1182-1183.

LeFevre ML; US Preventive Services Task Force. Screening for vitamin D deficiency in adults: US Preventive Services Task Force recommendation statement. Ann Intern Med. 2015;162(2):133-140. PMID: 25419853 pubmed.ncbi.nlm.nih.gov/25419853/.

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Review Date: 5/13/2020  

Reviewed By: Brent Wisse, MD, board certified in Metabolism/Endocrinology, Seattle, WA. Also reviewed by David Zieve, MD, MHA, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.

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