Methemoglobinemia - methylene blue test
The methylene blue test is a test to determine the type or to treat methemoglobinemia, a blood disorder.
The health care provider wraps a tight band or blood pressure cuff around your upper arm. The pressure causes veins below the area to fill with blood.
The arm is cleaned with a germ killer (antiseptic). A needle is placed into your vein, usually near the inside of the elbow or back of the hand. A thin tube, called a catheter, is placed into the vein. (This may be called an IV, which means intravenous). While the tube stays in place, the needle and tourniquet are removed.
A dark green powder called methylene blue goes through the tube into your vein. The provider looks at how the powder turns a substance in the blood called methemoglobin into normal hemoglobin.
No special preparation is required for this test.
When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing or slight bruising. This soon goes away.
There are several types of oxygen-carrying proteins in the blood. One of them is methemoglobin. Normal methemoglobin levels in blood are usually 1%. If the level is higher, you can become sick because the protein is not carrying oxygen. This can make your blood look brown instead of red.
Methemoglobinemia has several causes, many of which are genetic (problem with your genes). This test is used to tell the difference between methemoglobinemia caused by the lack of a protein called cytochrome b5 reductase and other types that are passed down through families (inherited). Your doctor will use the results of this test to help determine your treatment.
Normally, methylene blue quickly lowers the level of methemoglobin in the blood.
Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your provider about the meaning of your specific test results.
You may have a rare form of methemoglobinemia if this test does not significantly lower the blood level of methemoglobin.
There is little risk involved with having your blood taken. Veins and arteries vary in size from one person to another and from one side of the body to the other. Inserting an IV may be more difficult for you or your child than for other people.
Other risks associated with this type of blood test are minor, but may include:
Chernecky CC, Berger BJ. Methemoglobin - blood. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. St Louis, MO: Elsevier Saunders; 2013:781-782.
Neerja V, Graham S, Bem S. Basic examination of blood and bone marrow. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 31.
BACK TO TOPReview Date: 10/13/2022
Reviewed By: Anna C. Edens Hurst, MD, MS, Associate Professor in Medical Genetics, The University of Alabama at Birmingham, Birmingham, AL. Review provided by VeriMed Healthcare Network. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.
Health Content Provider
06/01/2025
|
A.D.A.M., Inc. is accredited by URAC, for Health Content Provider (www.urac.org). URAC's accreditation program is an independent audit to verify that A.D.A.M. follows rigorous standards of quality and accountability. A.D.A.M. is among the first to achieve this important distinction for online health information and services. Learn more about A.D.A.M.'s editorial policy, editorial process and privacy policy. A.D.A.M. is also a founding member of Hi-Ethics. This site complied with the HONcode standard for trustworthy health information from 1995 to 2022, after which HON (Health On the Net, a not-for-profit organization that promoted transparent and reliable health information online) was discontinued. |
The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. © 1997- 2024 A.D.A.M., a business unit of Ebix, Inc. Any duplication or distribution of the information contained herein is strictly prohibited.