A testosterone test measures the amount of the male hormone, testosterone, in the blood. Both men and women produce this hormone.

The test described in this article measures the total amount of testosterone in the blood. Much of the testosterone in the blood is bound to a protein called sex hormone binding globulin (SHBG). Another blood test can measure the "free" testosterone. However, this type of test is often not very accurate.

Serum testosterone

A blood sample is taken from a vein. The best time for the blood sample to be taken is between 7 a.m. and 10 a.m. A second sample is often needed to confirm a result that is lower than expected.

The health care provider may advise you to stop taking medicines that may affect the test.

You may feel a slight prick or sting when the needle is inserted. There may be some throbbing afterward.

This test may be done if you have symptoms of abnormal male hormone (androgen) production.

In males, the testicles produce most of the testosterone in the body. Levels are most often checked to evaluate signs of abnormal testosterone such as:

• Early or late puberty (in boys)
• Infertility, erectile dysfunction, low level of sexual interest, or thinning of the bones (in men)

In females, the ovaries produce most of the testosterone. The adrenal glands can also produce too much of other androgens that are converted to testosterone. Levels are most often checked to evaluate signs of higher testosterone levels, such as:

• Acne, oily skin
• Change in voice
• Decreased breast size
• Excess hair growth (dark, coarse hairs in the area of the moustache, beard, sideburns, chest, buttocks, inner thighs)
• Increased size of the clitoris
• Irregular or absent menstrual periods
• Male-pattern baldness or hair thinning

Normal measurements for these tests:

• Male: 300 to 1,000 nanograms per deciliter (ng/dL) or 10 to 35 nanomoles per liter (nmol/L)
• Female: 15 to 70 ng/dL or 0.5 to 2.4 nmol/L

The examples above are common measurements for results for these tests. Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different specimens. Talk to your provider about the meaning of your specific test results.

Certain health conditions, medicines, or injury can lead to low testosterone. The testosterone level also naturally drops with age. Low testosterone can affect sex drive, mood, and muscle mass in men.

Decreased total testosterone may be due to:

• Chronic illness
• The pituitary gland does not produce normal amounts of some or all of its hormones
• Problem with areas of the brain that control hormones (hypothalamus)
• Low thyroid function
• Delayed puberty
• Diseases of the testicles (trauma, cancer, infection, immune, iron overload)
• Benign tumor of the pituitary cells that produce too much of the hormone prolactin
• Too much body fat (obesity)
• Sleep problems (obstructive sleep apnea)
• Chronic stress from too much exercise (overtraining syndrome)

Increased total testosterone level may be due to:

• Resistance to the action of male hormones (androgen resistance)
• Tumor of the ovaries
• Cancer of the testes
• Congenital adrenal hyperplasia
• Taking medicines or drugs that increase testosterone level (including some supplements)

Chen Z, Legro RS, Ehrmann DA, Wei D. Androgen excess disorders in women. In: Robertson RP, ed. DeGroot's Endocrinology: Basic Science and Clinical Practice. 8th ed. Philadelphia, PA: Elsevier; 2023:chap 124.

Haddad NG, Eugster EA. Endocrinology of pubertal disorders. In: Robertson RP, ed. DeGroot's Endocrinology: Basic Science and Clinical Practice. 8th ed. Philadelphia, PA: Elsevier; 2023:chap 103.

Swerdloff RS, Wang C. The testis and male hypogonadism, infertility, and sexual dysfunction. In: Goldman L, Cooney KA, eds. Goldman-Cecil Medicine. 27th ed. Philadelphia, PA: Elsevier; 2024:chap 216.