BACK TOTOP Browse A-ZSearchBrowse A-ZABCDEFGHIJKLMNOPQRSTUVWXYZ0-9 E-mail FormEmail ResultsName:Email address:Recipients Name:Recipients address:Message: Print-FriendlyBookmarksbookmarks-menuCortisol urine test24-hour urinary free cortisol (UFC)The cortisol urine test measures the level of cortisol in the urine. Cortisol is a glucocorticoid (steroid) hormone produced by the adrenal gland.Adrenal glandThe adrenal glands are two small triangle-shaped glands in the upper abdomen. One gland is located on top of each kidney.ImageRead Article Now Book Mark Article Cortisol can also be measured using a blood or saliva test.BloodThe cortisol blood test measures the level of cortisol in the blood. Cortisol is a steroid (glucocorticoid or corticosteroid) hormone produced by th...Read Article Now Book Mark Article How the Test is Performed A 24-hour urine sample is needed. You will need to collect your urine over 24 hours in a container provided by the laboratory. Your health care provider will tell you how to do this. Follow instructions exactly.Collect your urine over 24 hoursThe urine 24-hour volume test measures the amount of urine produced in a day. The amounts of creatinine, protein, and other chemicals released into ...ImageRead Article Now Book Mark Article Because cortisol production by the adrenal gland can vary, the test may need to be done three or more separate times over a few weeks to months to get a more accurate picture of average cortisol production. How to Prepare for the Test You may be asked not to do any vigorous exercise the day before the test.You may also be told to temporarily stop taking medicines that can affect the test, including:Anti-seizure medicines Estrogen Human-made (synthetic) glucocorticoids, such as hydrocortisone, prednisone, and prednisolone Androgens How the Test will Feel The test involves only normal urination. There is no discomfort. Why the Test is Performed The test is done to check for increased or decreased cortisol production. Cortisol is a glucocorticoid (steroid) hormone released from the adrenal gland in response to adrenocorticotropic hormone (ACTH). This is a hormone released from the pituitary gland in the brain. Cortisol affects many different body systems. It plays a role in:ACTHThe ACTH test measures the level of adrenocorticotropic hormone (ACTH) in the blood. ACTH is a hormone released from the pituitary gland at the base...ImageRead Article Now Book Mark Article Bone growth Blood pressure control Immune system function Metabolism of fats, carbohydrates, and protein Nervous system function Stress responseDifferent diseases, such as Cushing syndrome and Addison disease, can lead to either too much or too little production of cortisol. Measuring urine cortisol levels can help diagnose these conditions.Cushing syndromeCushing syndrome is a disorder that occurs when your body has a high level of the hormone cortisol.ImageRead Article Now Book Mark Article Addison diseaseAddison disease is a disorder that causes the adrenal glands to not produce enough hormones.ImageRead Article Now Book Mark Article Normal Results Normal range is less than 45 mcg/24 hours (124 nmol/24 hours) for women and less than 60 mcg/24 hours (165 nmol/24 hours).Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or may test different specimens. Talk to your provider about the meaning of your specific test results. What Abnormal Results Mean A higher than normal level may indicate:Cushing disease, in which the pituitary gland makes too much ACTH because of excess growth of the pituitary gland or a tumor in the pituitary gland Cushing diseaseCushing disease is a condition in which the pituitary gland releases too much adrenocorticotropic hormone (ACTH). The pituitary gland is an organ of...ImageRead Article Now Book Mark Article Ectopic Cushing syndrome, in which a tumor outside the pituitary or adrenal glands makes too much ACTH Ectopic Cushing syndromeEctopic Cushing syndrome is a form of Cushing syndrome in which a tumor outside the pituitary gland produces a hormone called adrenocorticotropic hor...ImageRead Article Now Book Mark Article Severe depression Tumor of the adrenal gland that is producing too much cortisol Tumor of the adrenal glandCushing syndrome due to adrenal tumor is a form of Cushing syndrome. It occurs when a tumor of the adrenal gland releases excess amounts of the horm...ImageRead Article Now Book Mark Article Severe stress Rare genetic disordersA lower than normal level may indicate:Addison disease in which the adrenal glands do not produce enough cortisol Hypopituitarism in which the pituitary gland does not signal the adrenal gland to produce enough cortisol HypopituitarismHypopituitarism is a condition in which the pituitary gland does not produce normal amounts of some or all of its hormones.ImageRead Article Now Book Mark Article Suppression of normal pituitary or adrenal function by glucocorticoid medicines including pills, skin creams, eyedrops, inhalers, joint injections, chemotherapy Risks There are no risks with this test.Open ReferencesReferencesGuber HA, Oprea M, Russell YX. Evaluation of endocrine function. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 25.Newell-Price JDC, Auchus RJ. The adrenal cortex. In: Melmed S, Auchus RJ, Goldfine AB, Koenig RJ, Rosen CJ, eds. Williams Textbook of Endocrinology. 14th ed. Philadelphia, PA: Elsevier; 2020:chap 15.AllVideoImagesTogFemale urinary tract - illustration The female and male urinary tracts are relatively the same except for the length of the urethra.Female urinary tractillustrationMale urinary tract - illustration The male and female urinary tracts are relatively the same except for the length of the urethra.Male urinary tractillustrationFemale urinary tract - illustration The female and male urinary tracts are relatively the same except for the length of the urethra.Female urinary tractillustrationMale urinary tract - illustration The male and female urinary tracts are relatively the same except for the length of the urethra.Male urinary tractillustration Tests for Cortisol urine test Cortisol urine testUrine chemistryDexamethasone suppression testCortisol blood test17-hydroxycorticosteroids urine testACTH stimulation testRelated Information ACTH blood test(Medical Test)Cushing syndrome(Condition)Addison disease(Condition)Hypopituitarism(Condition)Congenital adrenal hyperplasia(Condition)Cushing syndrome due to adrenal tumor(Condition) Review Date: 5/13/2023 Reviewed By: Sandeep K. Dhaliwal, MD, board-certified in Diabetes, Endocrinology, and Metabolism, Springfield, VA. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team. The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. No warranty of any kind, either expressed or implied, is made as to the accuracy, reliability, timeliness, or correctness of any translations made by a third-party service of the information provided herein into any other language. © 1997- A.D.A.M., a business unit of Ebix, Inc. Any duplication or distribution of the information contained herein is strictly prohibited. © 1997- All rights reserved. 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Cortisol urine test24-hour urinary free cortisol (UFC)The cortisol urine test measures the level of cortisol in the urine. Cortisol is a glucocorticoid (steroid) hormone produced by the adrenal gland.Adrenal glandThe adrenal glands are two small triangle-shaped glands in the upper abdomen. One gland is located on top of each kidney.ImageRead Article Now Book Mark Article Cortisol can also be measured using a blood or saliva test.BloodThe cortisol blood test measures the level of cortisol in the blood. Cortisol is a steroid (glucocorticoid or corticosteroid) hormone produced by th...Read Article Now Book Mark Article How the Test is Performed A 24-hour urine sample is needed. You will need to collect your urine over 24 hours in a container provided by the laboratory. Your health care provider will tell you how to do this. Follow instructions exactly.Collect your urine over 24 hoursThe urine 24-hour volume test measures the amount of urine produced in a day. The amounts of creatinine, protein, and other chemicals released into ...ImageRead Article Now Book Mark Article Because cortisol production by the adrenal gland can vary, the test may need to be done three or more separate times over a few weeks to months to get a more accurate picture of average cortisol production. How to Prepare for the Test You may be asked not to do any vigorous exercise the day before the test.You may also be told to temporarily stop taking medicines that can affect the test, including:Anti-seizure medicines Estrogen Human-made (synthetic) glucocorticoids, such as hydrocortisone, prednisone, and prednisolone Androgens How the Test will Feel The test involves only normal urination. There is no discomfort. Why the Test is Performed The test is done to check for increased or decreased cortisol production. Cortisol is a glucocorticoid (steroid) hormone released from the adrenal gland in response to adrenocorticotropic hormone (ACTH). This is a hormone released from the pituitary gland in the brain. Cortisol affects many different body systems. It plays a role in:ACTHThe ACTH test measures the level of adrenocorticotropic hormone (ACTH) in the blood. ACTH is a hormone released from the pituitary gland at the base...ImageRead Article Now Book Mark Article Bone growth Blood pressure control Immune system function Metabolism of fats, carbohydrates, and protein Nervous system function Stress responseDifferent diseases, such as Cushing syndrome and Addison disease, can lead to either too much or too little production of cortisol. Measuring urine cortisol levels can help diagnose these conditions.Cushing syndromeCushing syndrome is a disorder that occurs when your body has a high level of the hormone cortisol.ImageRead Article Now Book Mark Article Addison diseaseAddison disease is a disorder that causes the adrenal glands to not produce enough hormones.ImageRead Article Now Book Mark Article Normal Results Normal range is less than 45 mcg/24 hours (124 nmol/24 hours) for women and less than 60 mcg/24 hours (165 nmol/24 hours).Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or may test different specimens. Talk to your provider about the meaning of your specific test results. What Abnormal Results Mean A higher than normal level may indicate:Cushing disease, in which the pituitary gland makes too much ACTH because of excess growth of the pituitary gland or a tumor in the pituitary gland Cushing diseaseCushing disease is a condition in which the pituitary gland releases too much adrenocorticotropic hormone (ACTH). The pituitary gland is an organ of...ImageRead Article Now Book Mark Article Ectopic Cushing syndrome, in which a tumor outside the pituitary or adrenal glands makes too much ACTH Ectopic Cushing syndromeEctopic Cushing syndrome is a form of Cushing syndrome in which a tumor outside the pituitary gland produces a hormone called adrenocorticotropic hor...ImageRead Article Now Book Mark Article Severe depression Tumor of the adrenal gland that is producing too much cortisol Tumor of the adrenal glandCushing syndrome due to adrenal tumor is a form of Cushing syndrome. It occurs when a tumor of the adrenal gland releases excess amounts of the horm...ImageRead Article Now Book Mark Article Severe stress Rare genetic disordersA lower than normal level may indicate:Addison disease in which the adrenal glands do not produce enough cortisol Hypopituitarism in which the pituitary gland does not signal the adrenal gland to produce enough cortisol HypopituitarismHypopituitarism is a condition in which the pituitary gland does not produce normal amounts of some or all of its hormones.ImageRead Article Now Book Mark Article Suppression of normal pituitary or adrenal function by glucocorticoid medicines including pills, skin creams, eyedrops, inhalers, joint injections, chemotherapy Risks There are no risks with this test.Open ReferencesReferencesGuber HA, Oprea M, Russell YX. Evaluation of endocrine function. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 25.Newell-Price JDC, Auchus RJ. The adrenal cortex. In: Melmed S, Auchus RJ, Goldfine AB, Koenig RJ, Rosen CJ, eds. Williams Textbook of Endocrinology. 14th ed. Philadelphia, PA: Elsevier; 2020:chap 15.